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These observations highlight differences between the immunogenicity of multivalent recombinant protein nanoparticles where immunogens are expressed, purified, and characterized ex vivo prior to delivery of a fixed antigen dose and repRNA, which enables streamlined manufacturing, but the final antigen dose depends on in vivo expression

For this purpose, some technologies for identification and confirmation of drug targets should be carried out

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The two-way orthogonal partial least squares (O2PLS) analysis, bolstered by loading value assessments, further uncovered the most significant role of Lactobacillus in determining metabolic profiles, being followed by Dialister , Hoylesella and Prevotella (Table S5)