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Protease assays Cells were collected on a glass filter (GF/A, 47 mm in diameter, Whatman) from 3 mL of culture, transferred to a Teflon-homogenizer and homogenized with 1 mL of 0.1 M acetate-NaOH (pH 5.0) buffer containing 28 mM 2-mercaptoethanol.Citation 40 The homogenate was centrifuged at 15,000 rpm for 10 min

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