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Yet, considering the importance of mitochondrial dysfunction in COPD and the increasing evidence highlighting mtDNA gene expression and methylation as potential biomarkers in a variety of diseases related to mitochondrial dysfunction and ageing, future studies may explore the impact of age and smoking status of COPD patients to provide a deeper understanding on mtDNA methylation and gene expression

Helen (University of California San Francisco, USA), for critical review of the manuscript and Tyson Douglass (University of California San Francisco, USA) for editorial assistance

9.11.3 The temperature of the flame positioned 250 6.4 mm above the burner tip shall be 800 50C

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