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In fact, the viability of the samples was maintained better in a nicotine-free environment indicating that even though vaping considerably increased the cytotoxic potential of the e-cigarette liquid, much of this cytotoxic effect was dependent on the volume of nicotine

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Deletion of Nrf2 abrogated the protective effects of AZI in vitro and in vivo To further confirm the role of Nrf2 in AZI-ameliorating airway epithelial barrier dysfunction, lentiviral-delivered shRNAs was used to knock down Nrf2 expression in HBECs

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