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One explanation lies in addiction persistence, wherein the physiological dependence on nicotine and the ingrained behavioural patterns developed over years of use make cessation difficult even after the onset of disease [3]

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Optimized donor RNA features, including structured 5 ends, shortened 3 UTRs and minimal homology requirements 60,61 , were sufficient to support TPRT in planta, indicating that R2-mediated insertion does not require extensive homology arms