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Previously amino acid changes have been introduced into key regions of the TMV CP that are required for appropriate self-assembly into the virus structure, and it was found that although some amino acid substitutions disrupted virion structures, they did not compromise virus movement and development of systemic symptoms ( Previously It has previously been demonstrated that protein and organic acid mediated synthesis of nanoparticles often leads to direct incorporation of these organic components into the nanoparticle during nucleation and growth phases ( Traditionally WT TMV has been used for production of metallized virus structures

However, he could not detect the pathogen microscopically and came to the conclusion that the pathogen was probably a small bacterium, while also noting that it could be a soluble enzyme-like blemish.

Davis, R., Rizwani, W., Banerjee, S., Kovacs, M., Haura, E., Coppola, D., & Chellappan, S