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You want to know who that patient is before you start prescribing anything, says Amy Killen, M.D., one of Blokes clinicians

The updated FAQs are more than reiteration, however

Total proteins were extracted in protein extraction buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM DTT, 0.5% (w/v) Nonidet P-40, and proteinase inhibitor cocktail] and centrifuged once at 6000 g, 4 C, for 20 min and twice at 17,000 g , 4 C for 10 min