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For heated hookah flavor volatiles collection, 0.5 g of hookah material was mixed with 0.5 g heated charcoal inside SPME screw cap vials (20 ml) placed on a hot plate kept at 190 C for 10 minutes followed by the addition of 2 ml distilled water

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Importantly, no significant change in mtDNA copy number was induced by CSE exposure during 25-week culture (Fig