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Smoking and T Levels: What Happens After You Quit Smoking Does Smoking or Nicotine Affect Testosterone Levels? Nicotine Blood, Urine, and Saliva Tests: What to Expect Does smoking increase testosterone? Both total and free testosterone levels increased significantly with increasing number of cigarettes smoked daily (p < 0.001). Smoking men had 15% higher total and 13% higher free Does cigarette smoking increase testosterone levels?
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