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Quantification of cell number was performed using ImageJ ( Quantification of immunofluorescence stained HBE cells For each sample, 3 random fields were selected for cell counting of DAPI + total cells, MUC5AC + goblet cells, TP63 + basal cells, CDH1 + epithelial cells, acetylated - Tubulin + ciliated cells and SCGB1A1 + club cells

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Picking up littered cigarette butts costs schools, businesses, and park agencies money, she says

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The WHO estimates that between 340 million and 680 million kilograms of tobacco waste are thrown away every year, and cigarette butts account for 30% to 40% of all items collected in coastal and urban clean-ups