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Its performance characteristics include: Glutathione coupled to Sepharose High Performance, excellent for high resolution purification of GST-tagged proteins Easy one-step purification of GST-tagged proteins and other glutathione S-transferase or glutathionedependent proteins Prepacked GSTrap HP columns for simple operation with a syringe, pump or chromatographic system, such as KTAdesign These features make Glutathione Sepharose High Performance the ideal medium for high resolution purification of GST-tagged proteins, glutathione S-transferases and glutathione binding proteins

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22 HustertE.HaberlM.BurkO.WolboldR.HeY

For all comparisons, the estimated differences or fold changes were reported alongside the 95 % family-wise CI and single-step adjusted P -values