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To confirm that the gut microbes were indeed major regulators of weight gain in the smoking cessation mice, the researchers collected microbiomes from smoking or smoking-cessation mice at various timepoints, and transferred these into germ-free mice that had never been exposed to cigarette smoke

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Given that the carbonic anhydrase inhibitor ACTZ blocks the intracellular production of bicarbonate ions and the depolarizing bicarbonate ion efflux, we hypothesized that ACTZ would prevent the diazepam-induced sIPSC frequency increase following nicotine exposure