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The An1b protein coding region was amplified by PCR using An1b-F and An1b-R (primer sequences are listed in Supplementary Table S2) and cloned into the pGADT7 vector using the ClonExpress II One Step Cloning Kit (Vazyme, China) to generate pGADT7-An1b

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Like many of those who ran before her, Shakur claimed her freedom only at a devastating cost: It meant relinquishing the ability to raise her only child

A 2019 law brought e-cigarettes and vape products into the tax base, driving a spike in collections that peaked in 2022 before declining