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The membrane was blocked with 5% skim milk for 1 h at room temperature and incubated with primary antibodies Bax, Bcl-2, Keap1, Nrf2, ZO-1, E-cadherin (1:1000 dilution) overnight at 4 , followed by HRP-conjugated secondary antibodies for additional 1 h at 37

but here was something there was no doubt of." The phonograph made Edison a celebrity and he received enormous praise for his invention, with Joseph Henry, president of the National Academy of Sciences calling Edison "he most ingenious inventor in this country

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