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The expression signature of very long non-coding RNA in myalgic encephalomyelitis/chronic fatigue syndrome

10.1016/S1470-2045(09)70137-8 111 KarthikeyanS.HotiS

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After the treatment period, the culture medium of each well was replaced by 0.2 ml of calcein AM solution (Sigma-Aldrich) at 2 M in phosphate buffer [PBS 1X] and the culture plates were put into the incubator for 30 minutes before fluorescence reading using a Synergy H1 microplate reader (Biotek, Winooski, VT, USA