To isolate LPMCs, the tissues were digested in RPMI (Gibco) supplemented with 20% fetal bovine serum (FBS), 1 mg/mL collagenase IV, and 0.05 mg/mL DNase I at 37 C for 30 min
#12 Perhaps We need some Canadians to chime in on this one given this was specifically marketed in that country
(That's right) Yeah, and what they call work, digging in the dirt Gotta get it in the ground 'fore the rain come down To get paid, to get the girl In your 4 wheel drive (A country boy can survive) Yeah the boys 'round here Drinking that ice cold beer Talkin' 'bout girls, talkin' 'bout trucks Runnin' them red dirt roads out, kicking up dust The boys 'round here Sending up a prayer to the man upstairs Backwoods legit, don't take no shit Chew tobacco, chew tobacco, chew tobacco, spit Aw heck Red red red red red red red red red red redneck Well the boys 'round here, they're keeping it country Ain't a damn one know how to do the dougie (You don't do the dougie?) No, not in Kentucky But these girls 'round here yep, they still love me Yeah, the girls 'round here, they all deserve a whistle Shakin' that sugar, sweet as Dixie crystal They like that y'all and southern drawl And just can't help it cause they just keep fallin' For the boys 'round here Drinking that ice cold beer Talkin' 'bout girls, talkin' 'bout trucks Runnin' them red dirt roads out, kicking up dust The boys 'round here Sending up a prayer to the man upstairs Backwoods legit, don't take no shit Chew tobacco, chew tobacco, chew tobacco, spit Let me hear you say (Ooh let's ride) (Ooh let's ride) Down to the river side (Ooh let's ride...) Hey now girl, hop inside Me and you gonna take a little ride to the river Let's ride (That's right) Lay a blanket on the ground Kissing and the crickets is the only sound We out of town (Let's ride) Have you ever got down with a
Stimulant abuse can cause severe impairment in your emotional and physical well-being
The geologist was Jean de Charpentier
