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Collection of Tissue specimens and epithelial cell processing Tissue specimens from tumour-free central bronchi of 3 control subjects (mean age, 63 3.2) were placed in a tube with sterile MEM supplemented with FBS 10%(56 C, 30 min), 1% MEM (nonessential amino acids, EuroClone), 2 mM L-glutamine, gentamicin 500 g/mL, and fungizone 50 mg/mL at 37 C in water-jacketed incubator with a humidified 5% CO2 atmosphere, overnight

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(United Nations Publications, 2000)