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Ser Jacopo's portrayals of the pipes Vincent painted take their subject matter seriously, and Guidi's deep appreciation for art and the arts is immediately apparent even at first glance

Fifty e-cigarette puffs was selected as an optimal controlled dose as it approximated the dose of nicotine delivered by the cigarette used in the study (~0.8 mg) based on machine yields of the e-cigarettes determined previously using a standardized Canadian Intense puffing protocol [20, 21]

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