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A separate study found similar results suggesting an increase in MUC5AC protein levels in culture human bronchial epithelial cells exposed to PG:VG aerosols [37] as well as an increase gene expression for several inflammatory markers including cytokines interleukin-6, interleukin-8 and matrix metalloproteinase-9 (MMP-9), known to play a role in the pathogenesis of COPD [37]

With 600 puffs of autonomy, these Puffs which are closed devices ( without a filling system ) and non-refillable should be thrown away after use

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Geneva: World health Organization (2015)