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The thermal cycling steps were: 50 C for 2 min, 95 C for 10 min, and 40 cycles of 95 C for 15 s and 60 C for 1 min, and qPCR was performed on a Step-One-Plus real-time PCR machine (Applied Biosystems) using the StepOne Software v2.3

Initial Assessment Before any treatment, technicians assess: Type and extent of damage Value of equipment versus restoration cost Likelihood of successful restoration Data recovery needs for storage devices Power Management All smoke damage electronics should be disconnected from power immediately

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Singapore enforced a ban on displaying cigarette packs at point-of-sale on 1 August 2017 to reduce the exposure of non-smokers, especially the youth, to the advertising effect of tobacco product displays, as well as to encourage current smokers attempting to quit by minimising impulse purchases of tobacco products