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Avoid drinking any other fluids, such as juice, coffee, tea, or sports drinks, as these can affect the levels of certain substances in your blood and skew the results of the test

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Abbreviations AJ: Adherent junction AZI: Azithromycin BALF: Bronchoalveolar lavage fluid COPD: Chronic obstructive pulmonary disease CS: Cigarette smoke CSE: Cigarette smoke extract GCL: Glutamate cysteine ligase GS: Glutathione synthetase GST: Glutathione S-transferase HBECs: Human bronchial epithelial cells Keap1: Kelch-like ECH-associated protein-1 MSEA: Metabolite set enrichment analysis NC: Negative control Nrf2: Nuclear factor erythroid 2-related factor 2 PBECs: Primary bronchial epithelial cells PCA: Principal component analysis SD: Sprague Dawley shRNAs: Short-hairpin RNAs TBHQ: Tert-butylhydroquinone TEER: Transepithelial electronic resistance TJ: Tight junction TNF-: Tumor necrosis factor-alpha Vc: Vitamin C ZO-1: Zonula occludens-1 References Brandsma CA, Van den Berge M, Hackett TL, Brusselle G, Timens W