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Finally, the DNA was resuspended in endotoxin/DNase-free water and its concentration measured using a NanoDrop UV-Vis Spectrophotometer (Thermo Fisher Scientific), with the purified DNA stored at -20C

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I would never repeat the B12 test, but many do so annually, and get extremely high results

There are several overlapping mechanisms that can produce the same visible result

PMID 3704105