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Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded, while anti-MBP (Rat, BioRad MCA4095,1:1000) was used to detect MBP

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If you have concerns about your B12 levels, talk with a registered dietitian or your healthcare provider, said Heather Greenlee, ND, PhD, MPH, an American Society of Clinical Oncology (ASCO) expert and medical director of the Integrative Medicine Program at Fred Hutch