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The prepared lysates were diluted 1:5 in an assay buffer and fluorescence intensities were measured using a Varioskan Flash spectral scanning multimode reader (Thermo Fisher Scientific, Inc.)

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Routes of exposure: Following release of sarin into the air, people can be exposed through skin contact or eye contact

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MAM (mitochondria-associated membranes) in mammalian cells: lipids and beyond

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The Bradford method for protein quantitation

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Thus with the help of a glutathione assay, GSH/GSSG ratio is determined and the level of oxidative stress can be measured

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