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At a 1:5 ratio, the gel did not adequately solidify (data not shown), whereas polymerization was successful at ratios of 1:6 and lower

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To further analyze whether apoptosis would coexist with necrosis in APAP-induced hepatotoxicity, we detected APAP-induced apoptosis by TUNEL staining and investigated the possible role of Bcl-2 family proteins and caspase-3 under this pathophysiological condition