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10.3390/ph17010038 294 ZhangS.-J.LuoD.LiL.TanR.-R.XuQ.-Q.QinJ.et al (2017a)

Figure 2 We have previously shown that the permeability of colonic biopsies was significantly higher in patients with IBS compared to healthy subjects (228)

Global RNA synthesis was measured using 2 h pulse labelling with Alexa 488-labelled EU and detected using RNA Click-IT HCS kit (C10327, Invitrogen) following the manufacturers protocol and quantified by automatic detection and intensity measurement using Nikon Eclipse Ti fluorescent microscope and Elements software

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who suggested that acetaminophen ingestion depletes intracellular GSH stores, which then causes loss of feedback inhibition of -glutamylcysteine synthetase activity (Figures 3a and 3b).This increases production of -glutamylcysteine, which is partially converted to pyroglutamic acid