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Metabolites were extracted using ice-cold acetonitrile, methanol and water (40:40:20, 0.5% formic acid) and neutralized with ammonium bicarbonate (15%)

The proposed fluorescent nanoprobe exhibited an excellent sensitivity with the LOD of 0.1 gmL 1 and a good accuracy for detecting Adriamycin

Reconstitution should be performed using bacteriostatic water or appropriate laboratory solvents according to established research protocols

The tripeptide-copper complex glycyl-l-histidyl-l-lysine-Cu2+ stimulates matrix metalloproteinase-2 expression by fibroblast cultures

Resulting eluted fractions were then subjected to another round of SEC as before