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For cell lysates sample detection, caspase-3 activity in DCA treated cells were determined following the instructions of Caspase-3 Colorimetric Assay Kit (K106, Biovision, San Francisco, USA) with substrate Ac-DEVD-pNA and detected by a Synergy H1 Microplate Reader (Bio Tek, Winooski, USA)

Nat Rev Mol Cell Biol 25(1):4664

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Common regimens use microgram (mcg) ranges, daily to weekly schedules, and cycles of 4 to 12 weeks before reassessment