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To evaluate growth on solid media, in all cases -with the exception of the metE mutant, which only grew in the presence of B12 and, therefore, plating with and without B12 was performed from liquid cultures supplemented with B12- the liquid cultures grown without B12 were plated in solid 7H10-ADC plates supplemented with or without AdoCbl (10 g/mL) and/or L-Met (25 g/mL)

When a true vitamin B12 deficiency exists, serious issues can arise

They may test you for GI inflammation or bleeding, bacterial infection, and/or whether you have nutrient absorption issues

In any case, lab results that indicate elevated levels of vitamin B12 in the blood will almost inevitably lead to a phone call from your doctor