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Despite this partial loss of native phenotype associated with extended in vitro culture, there is a growing interest in the use of higher-passage chondrocytes for cartilage tissue engineering [67, 68]

Glutathione plays a key role in our cell detoxification processes, but as we age, our glutathione levels naturally decrease

However, these reactions invariably result in the generation of reactive oxygen species (ROS) compounds that are unstable by having final layer of electrons unpaired and that, in trying to stabilize itself sequester electrons from other biomolecules, making them also destabilizes and, therefore, is no longer able to perform their duties properly, thus altering the homeostasis and, ultimately, causing cell death

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