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As mitochondria produce energy, they naturally generate free radicals known as Reactive Oxygen Species (ROS), such as superoxide and hydrogen peroxide

For the non-targeted metabolite analysis, the tissue metabolites were extracted in 80% MeOH at a final tissue concentration of 50 mg/mL for 24 h at 80 C

LiuMJBaoSGlvez-PeraltaMPyleCJRudawskyACPavloviczREet al

Thus, our results suggest that the conformational flexibility of the EHL domain of OTU1 is crucial for K6-linked diubiquitin chain cleavage, and this conformational change in the EHL domain upon recognition of the ubiquitin moiety could be a unique characteristic of the Lot DUB family