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Protein expression was detected using an enhanced chemiluminescence (ECL) kit (NCM Biotech, Suzhou, China) and a ChemiDoc XRS System (Bio-Rad, CA, USA)

The developer, Metabolic Pharmaceuticals, ran several phase II trials in obese adults between roughly 2003 and 2007

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Dysregulation of this pathway has been implicated in a myriad of diseases such as cancers, neurodegenerative diseases, and metabolic disorders, making the IGF-1 signaling pathway a prime target to develop therapeutic and intervention strategies