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To charge RHEB with GTPS (Abcam) or GDP (Sigma-Aldrich), RHEB was first diluted in buffer (50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM TCEP, 5 mM EDTA) and then supplemented with GDP or GTPS at a 30-fold molar excess

Multiple preclinical studies demonstrated that chronic ethanol consumption alters GABA A receptor plasticity, leading to ethanol dependence (Olsen and Liang, 2017)

Balli D, Ustiyan V, Zhang Y, Wang IC, Masino AJ, Ren X, et al

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Finally, reticular pseudodrusen are extracellular deposits that accumulate in the subretinal space