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Next, an efficient method is required for lysing the cells

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Then, the cultures were diluted with fresh LB broth and incubated in a shaking water bath (130 rpm) at 37 C to the logarithmic growth phase (OD 600 = 0.2)

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doi: 10.1042/bj20021446 28 MiyazakiM.MiyazakiT.NishimuraT.HojoW.YamashitaT