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The digested tissues were washed three times in PBS, collected by centrifugation (800 g for 10 min), placed into tissue culture dishes (Falcon Labware, NJ, USA) and resuspended in high-glucose DMEM, supplemented with 10% FBS and 50 g/ml gentamycin, and maintained at 37 C with 5% CO 2

Gently incorporate the solvent into the DSIP vial, with the needle pointed against the vial's wall

RemingtonRBechtelCLarsenDSamarAPageRMorrellCet al

Human trials testing DSIP for various applications used doses ranging from 25100 nmol/kg intravenously, with no doses exceeding 21.4 mcg/kg daily

(J Med Chem 2021) characterized the C-20 fatty diacid spacer that extends the half-life