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Miniature excitatory postsynaptic currents (mEPSCs) from Layer 56 cells in the medial prefrontal cortex (mPFC) were recorded in whole-cell voltage-clamp mode (holding potential = 70 mV) using an internal solution with the following composition (in mM): 117 Cs-methanesulfonate, 15 CsCl, 8 NaCl, 10 TEA-Cl, 0.2 EGTA, 4 Na-ATP, 0.3 Na-GTP, and 10 HEPES, pH 7.3 adjusted with CsOH ( ~ 300 mOsm)

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These served for years as the most viable alternative for treating the disease, until it was discovered that it was vitamin B12 that reversed the symptoms