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however, a GSH/GSSG ratio of 100 has very different reducing power for a GSH of 10 mM and a GSSG of 100 M with an Eh GSH/GSSG of 264 mV vs

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22 HallmayerJ.ClevelandS.TorresA.PhillipsJ.CohenB.TorigoeT.et al

20 Primarily, NADPH provides reducing equivalents for fatty acid synthase (FASN), the main rate-limiting enzyme, to synthesize fatty acids with acetyl-CoA serving as a primer and malonyl-CoA as a two-carbon donor, 21,22 and provides the needed electrons for ironsulfur (Fe/S) protein assembly that participate in non-essential amino acid biosynthesis and lipoic acid synthesis, tRNA modification, DNA replication and repair, and telomere maintenance

qPCR was performed using SsoFast EvaGreen Supermixes (BioRad) and a Roche LightCycler 96