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Methods Female C57BL/6 mice were orally administered letrozole (LE) to induce PCOS and then randomly divided into groups receiving daily oral administration of 160 mg/kg NAC (PCOS + NAC group), 200 mg/kg metformin (PCOS + Met group), or 0.5% carboxymethyl cellulose (drug solvent) (pure PCOS group) for 12 days

Purine and thymidine are synthesized through single-carbon metabolism and salvage mechanisms fueled by serine, promoting T cell growth and survival [15, 80]

Marked mydriasis rather than miosis may be seen due to hypoxia in overdose situations

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