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L1-Red/ L1-CCA input phage (~ 10 11 ) and streptavidin-coated magnetic Dynabeads (50 L/ selection, prewashed with 1 mL PBS) were incubated with 3% (w/v) mPBS (volume made up to 500 L for phage, 1 mL for beads) for 1 h on a rotary mixer at room temperature and 20 RPM

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Proper titration, which requires accurate reconstitution, helps manage these by allowing gradual dose escalation rather than sudden jumps