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Carnitine levels are normally less depleted in secondary carnitine deficiency when compared to OCTN2-deficient patients, and therefore smaller doses of L-carnitine can restore the carnitine levels in a shorter period of time than in patients with primary deficiency [41]

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THP-1 cell culture: THP-1 monocytes were seeded at a density of 2 10 5 /ml and differentiated into macrophages in the presence of 100 nM Phorbol-12-myristate-13-acetate (PMA) (Calbiochem, # 524400) for 2 days in RPMI low glucose media in 6-well plates or 4-well chambered slides as described previously 44,165