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Our study revealed that miR-214 increased the enrichment levels of histone H3K29ac at the promoter regions of ACSL4 , SLC38A1 , and PRKAA2 , indicating an activated chromatin state

Proteins (50 g) from PMS or mitochondrial extract from ASA/NAC treated GSH-depleted HepG2 cells were separated on 12% SDS-PAGE and transferred on to nitrocellulose paper by Western blotting as described in the Materials and Methods

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In terms of mechanism, sorafenib blocks the import of cysteine by inhibiting SLC7A11, the subunit of cystine/glutamate antiporter system Xc , thereby triggering ferroptosis and ultimately killing HCC cells (Louandre et al., 2013)