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[DOI] [PMC free article] [PubMed] [Google Scholar] 43.Ortiz DI, Piche-Ovares M, Romero-Vega LM, Wagman J, Troyo A

Recent studies have shown that metformin promotes adult neurogenesis under normal physiological conditions and in various disease models [31,32,33]

People forget that these are not mutually exclusive, like meal frequency, If youre not eating a lot, intermittent fasting makes perfect sense

Then, lysates were heated with Laemmli sample buffer (final 1X composition: 20 mM Tris, pH 6.8, 2% sodium dodecyl sulfate (SDS), 6% glycerol, 1% -mercaptoethanol, 0.002% bromophenol blue) at 95 C for 5 min