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Subsequently, the sections were incubated for 30 min at room temperature with horseradish peroxidase-conjugated streptavidin solution, stained with DAB chromogenic reagent, and dehydrated twice with 95% ethanol for 5 min and then with 100% ethanol

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Unit and frequency rules include: Units: one unit of 90471 per date of service, with additional vaccines routed to 90472 rather than multiple 90471 units MUE: 1 unit per date of service, set by the NCCI Practitioner MUE table Global period: 90471 carries an XXX indicator, so no global surgical package applies Frequency: The ACIP immunization schedule and documented medical necessity govern how often vaccines are administered Vaccines for Children (VFC) Program Billing Rules The Vaccines for Children (VFC) program supplies free vaccines for eligible children through age 18

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