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Twenty-four hours after transfection, cells were treated with CBD or vehicle for additional 24 h before luciferase assays were performed using the Dual Glo Luciferase Assay system (Promega) with firefly luciferase-based reporter gene activity normalized to the RLuc control

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On the basis of available data, the 72-hour regimen of oral N -acetylcysteine is as effective as the 20-hour intravenous regimen described previously, and it may be superior when treatment is delayed