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The cDNA samples (20 ng) were used for RT-qPCR in a total volume of 10 L using SYBR Green Reagent (#04887352001, Roche Diagnostics, Meylan, France) and specific primers, on a LightCycler 96 Roche Instrument

The novel advances in the biochemical diagnostics of primary aldosteronism, including omics techniques, presented in this review may help to address the most emerging problems, increase the number of diagnosed patients and facilitate the choice of an optimal treatment

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Missing SNPs (due to low coverage in some genotypes) were imputed using BEAGLE version 4.1 ( k -values ranging from 1 to 10 were estimated by running 10 iterations per K with a different seed value for each iteration (seed = 1 to seed = 10)

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