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Animal toxicology studies on BPC-157 reveal no lethal dose in rats receiving up to 20 mg/kg intramuscularly

doi:10.2147/ijn.S241055 184

After washing three times for 15 min each with 0.3% Triton X-100 in PBS and three times for 1 min each with PBS, the optical clearing started by incubating the slices in 25% fructose for 4 h, continued in 50% fructose for 4 h, 70% fructose overnight, 100% fructose (100% wt/vol fructose, 0.5% 1-thioglycerol and 0.1 M phosphate buffer, pH 7.5) overnight, followed by a final overnight incubation in SeeDB solution (80.2% (wt/wt) fructose, 0.5% 1-thioglycerol and 0.1 M phosphate buffer) 63

These compounds differ in how they work, what they target, how long they stay active in the body, and what evidence exists to support their use

Hoechst 33342 fluorescence ( ex = 355 nm) was detected using the 450/50-nm bandpass filter