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Further analysis indicated that among the 19 overgrown bacterial families, 12 possessed the urease gene ( Alteromonadaceae , Clostridiaceae , Cellulomonadaceae , Enterobacteriaceae , Dermabacteraceae , Halomonadaceae , Micrococcaceae , Moraxellaceae , Methylococcaceae , Pseudomonadaceae , Polyangiaceae , and Xanthomonadaceae ), 5 harbored the uricase gene ( Dermabacteraceae , Cellulomonadaceae , Xanthomonadaceae , Micrococcaceae , and Polyangiaceae ) producing ammonia that is harmful to the intestinal epithelium, and 3 expressed the tryptophanase gene necessary for the conversion of tryptophan into indole, which is then metabolized by the liver into uremic toxins ( Enterobacteriaceae , Clostridiaceae , and Verrucomicrobiaceae ) (Wong et al., 2014)

doi:10.1016/j.bbagen.2012.10.012 Ballatori N, Krance SM, Notenboom S, Shi S, Tieu K, Hammond CL

COMPOSITIONS AND METHODS FOR TREATING SCHISTOSOMIASIS, 2022

Subchronic exposure (90 days) to Cannabis sativa alkaloid extracts also demonstrated complex and dose-dependent effects

For example, in the European Union, the welfare of cephalopods used for scientific purposes is regulated by the Directive 2010/63/EU (and its Annex III, as amended by Commission Delegated Directive EU 2024/1262)