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For lactate quantitation, samples (5 L, run in triplicate) were added to 115 L assay buffer containing 100 mM glycylglycine, 100 mM glutamate, 2 mM NAD, 1 U/mL lactate dehydrogenase, and 1 U/mL glutamate pyruvate transaminase, pH 8.5

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Ageing Res Rev (2013) 12(1):37690

The mechanism of cellular damage is proposed to involve the reduction of free iron to Fe 2+ , followed by a Fenton-type reaction with H 2 O 2 to produce HO , that then oxidizes lipids and proteins [33]

378 This barrier dysfunction further induces immune imbalance 379 and abnormal intestinal substances entering the circulation, 380 and then affects the CNS through the gut-brain axis (such as the vagus nerve pathway) or immune/metabolic pathways, 381 forming a vicious cycle between ENS and central abnormalities