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Briefly, protein extracts were prepared by homogenizing with a Polytron homogenizer (KINEMATICA AG, Switzerland) in lysis buffer containing: 20 mM HEPES (pH 7.9), 25% v/v glycerol, 0.42 M NaCl, 0.2 mM EDTA, 1.5 mM MgCl2, 0.5% v/v Nonidet P-40, 1 mM DTT, 1 mM Naf, 1 mM Na 3 VO 4 , and 1 complete protease inhibitor cocktail (Roche Diagnostics Ltd, Mannheim, Germany)

Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice

Following oral administration of 100 mg of flupirtine, clearance is 275 ml/min in healthy volunteers

Considering the abundance of Hb in RBCs (corresponding to 10 m M heme), the reaction of autoxidation of Hb is therefore the most abundant source of ROS in RBCs

Medesan C, Cianga P, Mummert M, Stanescu D, Ghetie V, Ward ES