acetyl l carnitine metabolism saturated fat TRANSPORT AND FATTY ACID OXIDATION Carnitine Acyl-carnitine Carrier | Encyclopedia

[PubMed] 21: Younis N, Broadbent DM, Harding SP, Vora JP

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The eluted proteins were loaded in a Superdex 200 gel-filtration column (GE Healthcare, UK) for further purification with elution buffer [20 mM Tris-HCl pH 8.0/200 mM NaCl/5% (v/v) glycerol/1 mM dithiothreitol (DTT)/2 mM MgCl 2 ]

Afterward, protein G Sepharose was washed three times with cold STET buffer and once with STE buffer, and eluted with Laemmli sample buffer (62.5 mM TrisHCl, 2% SDS, 5% 2-mercaptoethanol, 10% glycerol, and 0.5 mg/ml bromophenol blue, pH 6.8)

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